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Therefore, it would be useful to gain a global understanding of the differences in gene expression between the wild type and mutant strains
Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)
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