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Importantly, both classes exhibit glucose-dependent mechanisms, meaning their effects diminish as blood glucose normalises, substantially reducing the risk of hypoglycaemia compared to sulphonylureas or insulin
Direct approach versus precursor approach, depending on the targeted research model.
This was measured by using arachidonic acid and adenosine diphosphate as inducers of platelet aggregation, which were incubated with blood samples of the subjects for three minutes at 37C
The samples were then incubated at 37 C in endothelial cell growth basal medium (EBM) containing 0.1% bovine serum albumin (BSA), in the presence or absence of the active form of vitamin D, 1,25(OH) 2 D 3 (100 nM), for a duration of 12 h
Mutations were introduced by QuickChange PCR
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