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Narita, S
1:2500, Abcam, Cambridge, UK), Drp-1 (1:2000, Novus Biotechnology, Littleton, CO, USA), Opa-1 (1:2000, Novus Biotechnology, Littleton, CO, USA), LC3A/B (1:2000, Cell Signaling Technology, Danvers, MA, USA), Tom-20 (1:2000, Santa Cruz Biotechnology, Dallas, TX, USA) and MnSOD (1:2000, Millipore, Billerica, MA, USA), Pink-1 (1:1000, BioVision Incorporated, Milpitas, CA, USA), Fis-1 (1:500, Santa Cruz Biotechnology, Dallas, TX, USA) and Parkin (1:500, Cell Signaling Technology, Danvers, MA, USA) for overnight
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The total reaction mixture consisting of 1.5 ml sodium phosphate buffer (0.1 M, pH 7.5) and PVP (1% w/v) L-methionine (13 mM), enzyme extract (0.1 ml) with same amounts of NBT solution (2.25 mM), riboflavin (60 M), Na 2 CO 3 (1 M), EDTA (3 mM) and double-distilled water (1.0 ml, DDW)
Yang, R., Tan, X., Thomas, A
US$ 23.50
US$ 23.84
US$ 27.71
US$ 29.66
