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Then the membranes were incubated with anti -Tubulin (Proteintech Group, Wuhan, 80713-1-RR), anti HSPA1A (Proteintech Group, Wuhan, 10995-1-AP), anti PERK (Abmart, Shanghai, TP52759F), anti GRP78 (Proteintech Group, Wuhan, 67686-1-Ig), anti DDIT3 (Abmart, Shanghai, T56694F), anti COL3A1 (Proteintech Group, Wuhan, 68320-1-Ig), anti SMAD2 (Proteintech Group, Wuhan, 67343-1-Ig), anti SMAD7 (ABclonal, Wuhan, A12343), anti VEGFR2 (Proteintech Group, Wuhan, 26415-1-AP), anti--Actin (Proteintech Group, Wuhan, 20536-1-AP), anti P16 (Proteintech Group, Wuhan, 10883-1-AP), anti P21 (Proteintech Group, Wuhan, 103551-AP), anti GAPDH (Proteintech Group, Wuhan, 60004-1-Ig) overnight at 4 C
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The cells in control well were not treated with FOXO4-DRI, and the blank well containing the medium without cells were used to normalized
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In theory, this can be calculated by: Maximum Current Draw = Capacity x C-Rating Drawing more current than specified by the C-rating is not recommended, as the battery can overheat, increase internal resistance over time, shorten battery lifespan, or even cause thermal runaway (catching fire) in extreme cases
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