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PBMCs were purified from other blood components and the ficoll histopaque by washing twice with 1x phosphate-buffered saline (PBS)
Rotate the application site daily
EFSA J 11:168 Fusunyan RD, Quinn JJ, Ohno Y, Macdermott RP, Sanderson IR (1998) Butyrate enhances interleukin (IL)-8 secretion by intestinal epithelial cells in response to IL-1 and lipopolysaccharide
Subsequently, we performed mRNA stability assays by treating cells with actinomycin D and found that GGCT mRNA degradation rate was significantly accelerated under 0 mM Gln compared with 4 mM Gln, indicating that Gln regulates GGCT in a post-transcriptional manner (Fig
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