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The discussion of oligomerization (page 3-8) is described elsewhere in detail (including cited references) and can be somewhat summarize here
The mobile phase parameters were set according to the following gradient: 013 min of 80% to 20% of mobile phase B
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Dilution Math and Dosing Table The Formula Concentration (mg/mL) = Peptide amount (mg) / BAC water volume (mL) Draw volume (mL) = Desired dose (mg) / Concentration (mg/mL) Units on U-100 syringe = Draw volume (mL) x 100 Reconstitution Reference Table 5 mg/mL is the most common target because it keeps dose volumes between 0.1-1.0 mL for the entire clinical dose range (2.5-15 mg), which fits a standard 1 mL insulin syringe without going below volumes that are prone to dosing error
& Herzenberg, L
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