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Label as Untreated and Induced groups
Lysates were resolved on SDSPAGE, transferred onto nitrocellulose membranes and probed with various antibodies
In addition, a large number of studies have shown that PACs activate the Nrf2-HO-1 pathway and ameliorate CIRI, bringing a new direction for CIRI treatment ( geraniin by the middle cerebral artery occlusion-reperfusion (MCAO/R), and OGD/R models, as well as by neurological scoring assays, CCK8 assessment, TUNEL staining, detection of cells by flow cytometry, and Western blotting assessment ( ex vivo and in vivo Nrf2, HO-1 protein expression in a concentration-dependent manner in the tMCAO model ( geraniin may be closely related to the Nrf2/HO-1 signalling pathway, but further experimental elucidation is needed in the future
During these sensitive phases, the substances protective properties can influence the effectiveness of treatments or biological processes
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