Buffers used for the various steps in the solubility of IBs LSB-1: 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM Phenylmethanesulfonyl fluoride (PMSF) (Sigma, Catalogue-P7626) and 500 L BugBuster Protein Extraction Reagent (70,584 Merck-Millipore)
Small tattoos can take 15-30 minutes, but a more intricate one may take several hours or days
Aliment Pharmacol Ther 2006;25:19-37
The most common are digestive issues, including: Nausea (the most common) Vomiting Changes in bowel movements (constipation or diarrhea) These usually improve over time
I don't know if his lab's still active, but he was at UC Irvine for a while, and then I think at University of Arizona as well