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Mice were assigned to respective study groups to comprehensively evaluate multiple medical weight loss approaches with various controls: 1) vehicle (Veh, sterile water) as control, 2) SEMA to induce weight loss using semaglutide, 2) WM-CR to induce weight loss by caloric restriction to match weight loss in SEMA, 3) continuous RETA to induce weight loss, or 4) short-term RETA to induce weight loss then regain, termed RETA withdrawal (RETA-w/d)