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Only oligopeptides in the trans conformation are able to bind to the active site of DPP4
This reduces anxiety about being caught unprepared
This allows more blood to flow through the vessels, which improves circulation
Injection Day 1 served as both the acute test and the first time point in the repeated-treatment timeline allowing evaluation of acute effects without requiring a separate acute-only experiment, minimizing animal use while preserving statistical power
Therefore, a formal Consent to Release Information form was not required
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