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The synthesis of creatine from glycine in the skeletal muscle of fish may promote more efficient coupling with local energy metabolism for physiological functions (e.g., constant swimming [32]) when compared with terrestrial mammals and birds, because guanidinoacetate is formed and further converted into creatine in the same muscle fiber

The mice were then treated with intraperitoneal injections of exogenous recombinant mouse IL-33 at 100 ng/mouse (PeproTech, Waltham, Massachusetts, USA) and/or ST2 neutralizing antibody at 10 g/mouse (R&D Systems, Minnesota, USA) every two days for 28 days following surgery

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