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Proteins in total homogenates (2040 g) or nuclear extracts (40 g) were resolved by 10% SDS-PAGE and immunoblotted using following primary antibodies: chicken polyclonal antibodies against mouse GCLM 7 and mouse ALDH1A1 60
(As described by OCallaghan and Fenech 2011)
One method is to divide the thigh into thirds lengthwise and widthwise
Add 2.66 mL BAC water
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