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Frozen cortical samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail

Regardless, the ability of 25HC to modulate integrin activation adds to its already extensive list of activities of 25HC in modulating various cellular responses 26,44,52,53,54,55

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