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Id randomly feel like I was going to be sick
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He XJ, Dai RX, Hu CL
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Lentiviruses were generated by co-transfection of each shRNA construct with envelope plus packaging vectors (pMD2.G, Addgene #12260 and psPAX2, Addgene #12259) and viral supernatants purified by ultracentrifugation onto 20% sucrose gradients
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