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Discarding the upper plasma, wash with three times volume of PBS for 3 times (re-suspend blood cell with PBS, centrifuge at 600 g for 10 minutes), add equal volume of Reagent I
The substantial Phase I loading dose aims to minimize this translational failure for TB-500
Adjustments were discussed clearly and I feel heard
Important Notice
The field of nanotoxicology lacks standardized guidelines, and the current efforts aim to adapt OECD Test Guidelines for Chemicals to be used for nanotoxicological properties [447]
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