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Most of the ATP in a cell is generated by the combined action of the TCA cycle and oxidative phosphorylation (OXPHOS) within the mitochondria 73,74

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To improve the selectivity, in this work, we design and synthesize a malononitrile-functionalized TPE derivative, TPE-DCV, which can undergo addition reaction with thiol group to the alkene bond, leading to the change in its fluorescence in the system (Figure 1)

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