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The -cyano amide-based Michael acceptors have also been elegantly used by the Taunton group as warheads in small-molecule inhibitors to react with active site cysteines while being minimally affected by the high concentration of intracellular GSH due to the fast reaction kinetics and the mM of K d (refs 31, 32)
Goat Anti-Mouse IgG H&L Alexa Fluor 594, Abcam, ab150116) diluted in 1% BSA in PBST for 1 h at room temperature
Anti-inflammatory Signalling Downregulates inflammatory cytokines at injury sites, supporting faster resolution of acute inflammation during the repair cascade
However, the chemical composition of GSH does not allow it to be taken orally for central nervous system diseases because little to none crosses the blood-brain barrier
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