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An antibiotic resistance cassette was PCR-amplified from either pFA6 natNT2 or pFA6 kanMX4 plasmids using primers designed to have 4050 base-pairs of homology directly up- and down-stream of the gene to be deleted

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Despite this shared mechanism, they differ significantly in receptor selectivity, GH pulse profile, and co-secreted hormones differences that substantially affect which compound is appropriate for different research questions

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