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Introducing steric blockade has proven a successful strategy to increase the stability of a peptide in vivo but typically this has been at the expense of potency, as for instance a 48-fold difference at human GLP-1R in transfected CHO cells between Ab#2_DSB#1 and the same molecule without the disulphide bridge (Ab#2_EX4)
doi: 10.18632/oncotarget.16113 86 ZhangJLiXHanXLiuRFangJ
Treat protein targets as a non-negotiable daily task, like taking the medication itself
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