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10.1016/j.cell.2009.11.020 Cell 35 JiaC

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Lyophilized: freeze at 20 C (4 F)

After 12 hours of thermal stress, 6 fish were randomly sampled from each tank, anesthetized with MS-222 (0.125 g/L), and dissected to collect liver tissues, which were then frozen in liquid nitrogen for subsequent analysis