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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice
Final Thoughts Cagrilintide represents a distinct branch of metabolic peptide research from the GLP-1/GIP/glucagon receptor agonist line that has dominated recent attention
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