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For instance, changes in DNA methylation patterns, histone modifications, as well as circulating miRNA expression alterations may affect critical biological pathways, thereby influencing health outcomes in NS workers 220
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Quantitative real-time polymerase chain reaction (qRT-PCR) To precisely evaluate the hepatic gene expression levels of AMPK, PI3K, AKT, IL-1, and HO-1, the technique of quantitative real-time polymerase chain reaction (qRT-PCR) was employed