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There was also an increase in GSH1 transcript level in the GSH-supplement condition, suggesting the possibility of pTEF1 regulation by relatively high ( 1 mM) extracellular GSH
The lysate was centrifuged at 13000 g at 4 C, and supernatant subjected to deproteinization procedure via TCA precipitation (Abcam
ChemBioChem 13 , 11161120 (2012)
Furthermore, the specific inhibition of NNMT by 5-amino-1mq avoids interference with other metabolic enzymes, ensuring the specificity and reliability of experimental results
Conclusions This pilot study demonstrates the potential therapeutic effects of oral administration of glutathione in practical dose for patients with NAFLD
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