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Western blots were visualized using ECL substrate (Yeasen, China), and protein bands were quantified using Gel-Pro-Analysis software

Sterile water, by contrast, has no such protection once opened, its generally recommended for immediate, single-use only, with any leftover discarded the same day

Mdzinarishvili A, Sutariya V, Talasila PK, Geldenhuys WJ, Sadana P