Cell culture The human melanoma cell lines SK-MEL-147 (Memorial Sloan Kettering Cancer Center) and WM88 (Rockland, Cat #WM88-01-0001), the glioblastoma cell line ONDA7 (JCBR, Cat #JCBR1575), the breast cancer cell line SK-BR-3 (ATCC, Cat #HTB-30), the human kidney cell line HEK293T (ATCC, Cat #CRL-3216), and the mouse melanoma cell line B16F10 (ATCC, Cat #CRL-6475) were cultured in high glucose DMEM (Gibco), fortified with 10% syringe-filtered FBS (Gibco), 1% penicillin/streptomycin (Gibco), and 0.2% Normocin (InvivoGen)
September 20, 2019
The tests for the multiple endpoints will follow the weighted Holm-Bonferroni procedure such that if 1 of the 2 endpoints is superior, then the full alpha can be recycled for the other endpoint, and, hence, the remaining primary endpoint will be tested at the 5% significance level (2-sided) (Figure 2)
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