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These cells secrete proinflammatory cytokines, including IFN-, IL-1, IL-12, CCL4, CCL5, and GM-CSF, which are associated with hepatocyte damage, increased ROS production, apoptosis, and activation of JAK-STAT1/3 and NF-B signaling pathways (109)
These regulatory effects may underlie observed improvements in sleep-wake patterns and neuroendocrine homeostasis in aged subjects [3]
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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)
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