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It tested a modified analog, run by a company called CohBar, in 88 volunteers over 28 days
The distribution of the bases within the transcripts was determined using GATKs CollectRnaSeqMetrics
PMID: 34789012 Chen, K., et al
To make the mutated binding site construct, four bases of the putative miR18a binding site on Gclc 3 UTR were mutated using sitedirected mutagenesis, as per manufacturer's instructions (QuikChange Lightning SiteDirected Mutagenesis Kit, Agilent)
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