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Temperature dependence of spontaneous transport kinetics Unbiased equilibrium simulations can provide quantitative predictions of the total solute flux across a lipid bilayer by counting the number of spontaneous transmembrane transitions

Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air

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