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To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin
Each batch is tested for identity, purity, and stability to ensure quality for controlled research environments
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Mann Whitney U-test, Kruskal Wallis test and Spearmans correlation test were all performed using the SPSS Statistics 20 software (IBM, Armonk, NY)
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