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I carried those biases with me into practice

RCR (Respiratory Control Ratio): Ratio of V3 to V4, a measure of coupling efficiency

FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay

An OA model was established by ACLT, as previously reported [9]