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Clin Biochem Rev 25(3):155164 Baeshen MN, Bouback TA, Alzubaidi MA, Bora RS, Alotaibi MA, Alabbas OT, Alshahrani SM, Aljohani AA, Munshi RA, Al-Hejin A, Ahmed MM, Redwan EM, Ramadan HA, Saini KS, Baeshen NA (2016) Expression and purification of C-peptide containing insulin using Pichia pastoris expression system