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Samples were sonicated on ice for 1 min using a VCX 130 probe sonicator (5 s on/off cycles), incubated for 30 min, and centrifuged at 17,000 g for 20 min at 4 C
For an extra boost, stir in a pinch of baking soda as well
Background: Aging is an ubiquitous biological phenomena characterized by ever increasing susceptibility to diseases, toxic effects of xenobiotic and an ultimate genome-instability and diminished DNA-damage-repair capacity and cell death which occurs in neurodegenerative diseases including PD
glyoxalases, which use GSH to detoxify reactive dicarbonyl species including methylglyoxal and glyoxal
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