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SDS-PAGE and Western blotting Samples were loaded onto 10% SDS-polyacrylamide gels then transferred to 0.45-m PVDF membranes and blocked with 5% skimmed milk [or 5% bovine serum albumin (BSA) for anti-phospho antibodies] in TBS buffer supplemented with 0.1% Tween 20 (TBS-T)
A series of trials in populations with T2D will also be undertaken
provided by Novo Nordisk, Copenhagen, Denmark) diluted in TBS containing 1% casein, 5% normal goat serum, and 0.1% NaN 3
In poorly managed T2DM treated with basal insulin, tirzepatide can improve glucose control without hypoglycemia risk, alone or in combination with other oral hypoglycemic medications [79]
Additionally, avoid sun exposure and tanning beds for a week prior to the treatment
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