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The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)

Adverse Effects They arent trivial, well beyond injection site swelling and redness

PMID: 34830181

We analyzed the elongation of free ubiquitin chains assembled either by UBR5 or TRIP12, to assess the activity of these E3s, irrespective of substrate properties

This educational protocol presents a subcutaneous injection approach to maximize bioavailability from the 10 mg vial format