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LCMS-Based Metabolomics Cells were processed using the LIMeX workflow, and polar metabolites were extracted with a biphasic solvent system composed of cold methanol, methyl tert-butyl ether, and 10% methanol, followed by liquid chromatography-mass spectrometry (LCMS) analysis [45]
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A dose of 7,500 mg/day of choline was found to have a slight blood pressure-lowering (hypotensive) effect, which could result in dizziness or fainting
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