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Another group generated isogenic hiPSC lines with engineered mutations in exon 2 and 8 of the DISC1 , leading to loss of long DISC1 isoforms and affecting NPC proliferation, baseline WNT signaling and the expression of NPC fate markers such as FOXG1 and TBR2 [143]
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A disadvantage of the procedure is that is peptides larger than 30 to 40 units do not give reliable results
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