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Aggregation: Peptides can clump together, forming inactive aggregates

Almost every patient who sits in my consultation room asks the same thing, usually followed by, Doctor, I dont want a short-term glow

Liu Y, Hou Q, Wang R, Liu Y, Cheng Z (October 2023)

FOXO4-DRI acts as a competitive inhibitor of this interaction binding directly to the transactivation domain 2 (TAD2) of p53, displacing FOXO4, and allowing phosphorylated p53 to be exported from the nucleus and directed to mitochondria, where it activates BAX, triggers caspase-3 cleavage (via the p53/BCL-2/Caspase-3 signalling pathway), and drives cell-intrinsic apoptosis

The math behind reconstitution is straightforward