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The priming phase is initiated when DAMPs or PAMPs engage receptors such as toll-like receptors (TLRs) or cytokine receptors, triggering the NF-B pathway to increase mRNA levels of NLRP3, pro-IL-1, and pro-IL-18 (Zahid et al., 2019)

Lipid blockade revealed by MD simulations To further evaluate the conformational state and to investigate protein-lipid interactions in Tc MscS, we performed three replicas of 100 ns all-atom MD simulations with or without pore lipids (see Methods for system preparation)

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Then the fluorescent secondary antibody was incubated at room temperature for 1 h