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The concentrations of proteins were determined with a BCA protein assay kit (Beyotime Institute of Biotechnology, Shanghai, China), and the proteins were boiled with 5X SDS-PAGE sample loading buffer (Beyotime Institute of Biotechnology, Shanghai, China)

But the subcutaneous tissue in your abdomen, thigh, and upper arm is threaded with a network of tiny capillaries

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Aliquot into single-use volumes and avoid repeated freeze-thaw cycles

Used to characterise the pharmacodynamic consequences of C18 fatty diacid-albumin binding on GLP-1R engagement kinetics, receptor activation amplitude and duration, biased agonism profiles across Gs, Gq/11, and beta-arrestin signalling pathways, receptor internalisation and intracellular trafficking dynamics, and GLP-1R recycling versus lysosomal degradation under chronic long-acting agonist exposure