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Spectrophotometric readings from assay mixture containing 0.2 M potassium phosphate buffer (pH 7.5), 0.1 mM EDTA, 0.5 mL of 3 mM DTNB, 0.1 mL of 2 mM NADPH, 0.1 mL of 2 mM GSSG and 0.1 mL supernatant [extracted in 100 mM potassium phosphate buffer (pH 7.5)] at 412 nm was used to calculate GR activity using extinction coefficient of 6.22 mM 1 cm 1

The groups were similar in composition