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Your muscles can begin to involuntarily contract if you push them beyond their normal limits without giving them time to rest
Sounds like a footnote
If your protocol requires strict dosing consistencyand reproducible research always doestreat any temperature deviation as a compromised batch
Suitable fractions were pooled, concentrated with Amicon Ultra 10 kDa filters (Merck Life Science Ltd, Gillingham, UK) and diluted in 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl and 5 M urea buffer for storage at -80 C
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