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doi: 10.3389/fimmu.2025.1591151 Received 11 March 2025 Accepted 30 June 2025 Published 17 July 2025 Volume 16 - 2025 Edited by Francisco Javier Snchez-Garca, National Polytechnic Institute (IPN), Mexico Reviewed by Jose L

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The protein was eluted with 0.1 M glycine-HCl (pH 2.0) and neutralized with 1 M Tris-HCl (pH 8.5)

[10] [11] Similarly, the SURMOUNT-4 trial showed significant weight regain after tirzepatide discontinuation

Abstract The incidence and prevalence of type 2 diabetes (T2D) are increasing worldwide, affecting both adults and a growing number of children and adolescents