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UMN culture is an important tool to investigate UMN responses to treatment in vitro UCHL-eGFP is a reporter mouse line for corticospinal motor neurons (CSMN, a.k.a the UMNs in mice), in which CSMN express eGFP, and CSMN identity of eGFP+ neurons in the layer 5 of the motor cortex is confirmed by retrograde labeling, molecular marker expression and electrophysiological properties 31
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