Anti-LC3B (D11), anti-GAPDH antibodies, and senescence -galactosidase staining kit were purchased from Cell Signaling Technology (Danvers, MA, USA)
GHK-Cu with retinol and vitamin C Skincare combinations: GHK-Cu + Retinol: Can use together but separate timing Morning: GHK-Cu topical Evening: Retinol OR alternate days Start slowly (every 3rd day retinol) GHK-Cu + Vitamin C: Compatible, can layer Apply vitamin C first Wait 5-10 minutes Apply GHK-Cu Or use different times of day Complete skincare protocol: Morning: Cleanse, vitamin C, GHK-Cu, moisturizer, SPF Evening: Cleanse, GHK-Cu, retinol (alternate days), moisturizer See our can you use peptides and retinol together and should copper peptides be refrigerated
Target: Reach about 5,333 mcg total (~20 units) daily by weeks 712
Roberts is in this camp
This dual-compound approach differs fundamentally from GLP-1 plus DPP-4 stacking, because tirzepatide itself is a GLP-1/GIP co-agonist rather than an enzyme inhibitor