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Particles: Floating specks or fibers mean contamination

Xing, Y., Ye, Y., Zuo, H., & Li, Y

The deficiency was more severe than expected People with advanced B12 deficiency, especially with neurological symptoms, often feel rough at the start of treatment because the underlying problem was significant

For example, depending on the assay, serum B12 cutoffs for deficiency for adults may range from 120 to 180pmol/L, with 148pmol/L based on 3SDs from the mean of an adult reference range, said to be the most common cutoff for frank deficiency (IOM, 2000

Hydrophilic compounds dissolve well in sterile water or bacteriostatic water, while acidic peptides may require basic buffers before dilution