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Briefly, muscles were collected, trimmed of connective tissue and fat, minced with scissors, then digested with 1.25 mg/ml of protease type XIV for 1 h at 37C

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The blocked membranes were incubated with the specific primary antibodies diluted in 5% BSA (Sigma-Aldrich) with 0.05% sodium azide overnight on a shaking bed at 4 C

Statistics by two-sided unpaired t-tests compared to sgControl-expressing cells (b, c, e, f) or empty vector transduced cells (h, i, k)