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For the maximum LDH release control group, 4 l of lysis solution (Promega, USA) was added into each well and incubated for 30 mins to initiate cell lysis prior to the next step

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Research published in Scientific Reports demonstrates that aged mice treated with NNMT inhibitors showed approximately 40% greater grip strength compared to untreated controls, remarkably surpassing even the benefits achieved through intensive exercise alone, which produced only 20% improvement