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To further explore the relationship between ferroptosis and fibrotic factors in HK-2 cells, we established a GPX4-overexpressing plasmid system in HK-2 cells and treated the cells with TGF- for 48 h (Fig

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T-Y.T was supported by Universiti Kebangsaan Malaysia postdoctoral research scheme

Peer review Peer review information Nature Reviews Drug Discovery thanks Betty Diamond and the other, anonymous, reviewers for their contribution to the peer review of this work

We used the GSH/GSSG-Glo Assay to monitor GSH changes in the cells dosed with BSO (Figure 5, Panel B)