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The membrane was then blocked with blocking buffer (1% BSA, 20 mM Tris-HCl [pH 7.5], and 1 mM dithiothreitol) containing 0.05% Tween 20 for 1 h at room temperature, incubated with 20 M His--syn recombinant protein in blocking buffer overnight at 4 C, and washed with washing buffer (10 mM Tris-HCl and 100 mM NaCl)

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However, these injections should be viewed as tools, not miracle solutions