Patients who treat their mood by utilizing comfort food could turn to other less desirable alternatives
Such risks and uncertainties include, but are not limited to: our ability to achieve our strategic and operational initiatives
SNAP/FLAG-hGLP-1R plasma membrane recycling INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto poly-L-lysine-coated black, clear bottom 96-well plates in full media, stimulated with vehicle or 100 nM exendin-4 for 1 hour at 37C to enable receptor internalisation, washed with PBS and incubated in 100 nM tetramethylrhodamine (TMR)-tagged exendin-4 (Ex-4-TMR) in full media for 1 hour or 3 hours at 37C to label any receptors that recycle back to the plasma membrane after agonist-mediated internalisation
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Janjigian YY, Smit EF, Groen HJ, Horn L, Gettinger S, Camidge DR, et al